Fig. 5. Evidence of p53 LLPS in a cell-based model. (a) Collection of confocal images showing the fluorescence and bright-field (BF) channels. EGFP-fused proteins were used as reporters to determine the localization of wt and M237I full-length p53 after the transfection of H1299 cells (scale bars, 5 μm). (b) Zoomed-in images of cropped areas in ‘a’ showing the nuclear body compartments (nucleolar regions) containing wt (red arrows) and M237I p53 (blue arrows). (c) DIC images of wt and M237I full-length p53 showing fluorescence recovery after photobleaching of the nuclei and nucleolar condensates. Red circle shows bleached area. White arrow shows nucleolar p53 before bleaching (scale bars, 20 μm). (d and e) Line plots showing fluorescence recovery of (d) wt and the M237I p53 and (e) M237I p53 in two different cellular compartments, the nucleus and nucleolus. The data are shown as the mean ± s.d. of n = 5 measurements.