Fig. 8. Heparin-induced p53C phase separation based on Congo Red binding under crowded conditions. (a and b) Fluorescence channels of EGFP and Congo Red (CR) (100 μM) and the bright field (BF) images of (a) wt and (b) M237I p53C in the absence (−) and in the presence (+) of PEG. (c) Fluorescent channels of EGFP and Congo Red (CR) and the bright field (BF) images of wt and M237I p53C at 4 °C after the addition of heparin. The inset shows the result obtained in the presence of 10 μM CR. (d) Fluorescence channels of EGFP and Congo Red (CR) and the bright field (BF) images of WT p53C at 15 °C in the absence (−) or in the presence (+) of heparin. Heparin was added before CR.