Fig. 2. (a) Long-time response of the microsensor polarized by a constant potential (black curve) and pulse potential (red curve). (b) Schematic illustration of ion concentration changes under different pulse potentials. (c) Current traces and calibration curves (error bars were obtained for the same micropipette from three independent measurements) obtained for the PvimB-modified micropipette towards the successive pH variation from 5.8 to 8.0 with different −Vstep. The electrolyte was 100 mM NaCl in 50 mM phosphate buffer (pH 5.8).
