. Author manuscript; available in PMC: 2021 Jul 1.

Published in final edited form as: Ticks Tick Borne Dis. 2021 Apr 9;12(4):101727. doi: 10.1016/j.ttbdis.2021.101727

Table 1.

PCR primers used for amplifying various gene segments of Ehrlichia and Anaplasma species in this study.

Name	Sequence	Annealing Temperature	Size (bp)	Reference(s)
EHR16SF	GGTACCYACAGAAGAAGTCC	55 °C	345	Inokuma et al., 2000; Parola et al., 2000
EHR16SR	TAGCACTCATCGTTTACAGC		345	Inokuma et al., 2000; Parola et al., 2000
Aplatys.16S-F	TTTGTCGTAGCTTGCTAT	50 °C	349	Matei et al., 2016
Aplatys.16S-R	CTTCTGTGGGTACCGTC		349	Matei et al., 2016
Aplatys-23sF2	TCGATGGGAATCAGGTTAATATTCCTG	55 °C	800	This study
Aplatys-23sR2	TTGTACTATAAAAGCTGATTTCCG		800	This study
Aplatys-groEF	AAGGCGAAAGAAGCAGTCTTA	54 °C	724	Inokuma et al., 2002
Aplatys-groER	CATAGTCTGAAGTGGAGGAC		724	Inokuma et al., 2002
Aplatys-gltAF	GACCTACGATCCGGGATTCA	61 °C	580	Silva et al., 2016
Aplatys-gltAR	CCGCACGGTCGCTGTT			Silva et al., 2016
E. canis F	CAATTATTTATAGCCTCTGGCTATAGGA	58 °C	475	Rufino et al., 2013
E. canis R	ATAGGGAAGATAATGACGGTACCTATA			Rufino et al., 2013