Fig. 4.
PRSS3 effect on the migration and invasion capabilities of HCC cells. a Cell monolayer scratching assay was performed to monitor the cell migration ability of HepG2 and PLC/PRF/5 cells with or without ectopic PRSS3 overexpression. b, c Cell movement ability of HepG2 and PLC/PRF/5 cells with or without PRSS3 overexpression was assessed using transwell migration assay (b) and invasion assay (c), respectively. Left panel: representative image; right panel: quantitative analysis. d Transwell migration and invasion assays of SUN387 cells transfected with siPRSS3–2 or siNC. Left panel: representative image; right panel: quantitative analysis. e Western blotting of the levels of MMP2 in the transfected HCC cell lines. Data from at least three times independent experiments is presented as the mean ± SD, *p < 0.05, versus control