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. Author manuscript; available in PMC: 2021 Jun 2.
Published in final edited form as: J Pathol. 2017 Sep 21;243(3):376–389. doi: 10.1002/path.4958

Figure 5.

Figure 5.

TLR4 in cancer cells mediates the cross-talk between autophagic CAFs and BCICs. (A) Correlation between ALDH1 and HMGB1 receptor (TLR4, TLR2, TLR9 and RAGE) mRNA levels in luminal breast cancer (n = 641, TCGA). Statistical significance was determined by linear regression and the F-test. (B) TLR4 and ALDH1A1 mRNA levels in TLR4+ and TLR4− MCF-7 cells. (C) The levels of POU5F1, SOX2 and NANOG in TLR4+ cells with or without 1 μg/ml HMGB1. (D) ALDH+ MCF-7 and T47D cells in CM-CAF3 with or without 30 nM TAK-242. (E) Western blots of POU5F1, SOX2 and NANOG in MCF-7 and T47D cells in CM-CAF3 with or without TAK-242 (30 nM). (F) Tumour volumes in mice with mammary pad injection of TLR4+ and TLR4− MCF-7 cells with or without CAF3 transfected with shCtrl or shATG5 measured 2 weeks after implantation (n= 5 mice per group). **P < 0.01.