Extended Data Fig. 3.

Diphtheria toxin (DT) administered every two days from day 8 to 14 after birth followed by quantitative analyses on day 15 of the absolute count of B cells (CD45⁺ CD19⁺, LysCre^+/−: n=5, MM^DTR n=5), neutrophils (CD45⁺ Ly6G⁺, LysCre^+/−: n=5, MM^DTR n=4), dendritic cells (DC) (CD45⁺, Lin⁻, CD11c^hi, MHCII⁺, CD64⁻, LysCre^+/−: n=5, MM^DTR n=4), eosinophils (CD45+, SiglecF⁺, LysCre^+/−: n=5, MM^DTR n=4) in the colon of control littermates LysCre^+/− or MM^DTR animals (A). DT administered every two days from day 8 to 14 after birth followed by quantitative analyses on day 42 of the absolute count of MAIT cells (CD45⁺ CD3ε⁺ TCRβ⁺ MR1/5-OP-RU Tetramer⁺) in the colon of control littermates LysCre^+/− (n=5) or MM^DTR (n=5) animals (B). Absolute count of macrophages (CD45⁺ Lin⁻ F4/80⁺ CD64⁺) (C), iNKT (CD45⁺ CD3ε⁺ TCRβ⁺ CD1d Tetramer⁺) and TCR-αβ⁺ T (CD45⁺ CD3ε⁺ TCRβ⁺) cells (D) in the colon in the absence of DT treatment in control littermates LysCre^+/− (n=4) and MM^DTR (n=4) animals at 2 weeks old. Absolute counts were determined by flow cytometry. Error bars indicate standard error of mean. Each dot is representative of an individual mouse. P values were calculated by unpaired two-sided Student’s t-test. ns: not-significant.