Extended Data Fig. 2.

Diphtheria toxin (DT) administered every two days from day 8 to 14 (DT8–14) after birth followed by quantitative analyses on day 15 (H15) of the absolute count of macrophages (CD45⁺ Lin⁻ F4/80⁺ CD64⁺) in the skin (LysCre^+/−: n=3, MM^DTR n=4) (A) or spleen (LysCre^+/−: n=5, MM^DTR n=5) (B) and the absolute count of iNKT (CD45⁺ CD3ε⁺ TCRβ⁺ CD1d Tetramer⁺) and TCR-αβ⁺ T (CD45⁺ CD3ε⁺ TCRβ⁺) cells in the skin (LysCre^+/−: n=8, MM^DTR n=7) (C) of control littermates LysCre^+/− or MM^DTR animals. DT administered from day 8 to 10 (DT8–10) after birth followed by quantitative analyses on day 11 (H11) of the absolute count of iNKT and TCR-αβ⁺ T cells in the small intestine (D) and lung (E) of control littermates LysCre^+/− (n=3) or MM^DTR (n=12) animals. DT administered from day 8 to 10 (DT8–10) after birth followed by quantitative analyses on day 11 (H11) of the absolute count of splenic macrophages (F) of control littermates LysCre^+/− (n=5) or MM^DTR (n=3) animals. DT administered from day 12 to 14 (DT12–14) after birth followed by quantitative analyses on day 15 (H15) of the absolute count of splenic macrophages (G) of control littermates LysCre^+/− (n=9) or MM^DTR (n=10) animals. Absolute counts were determined by flow cytometry. Error bars indicate standard error of mean. Each dot is representative of an individual mouse. P values were calculated by unpaired two-sided Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.