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. 2021 May 20;17(5):e1009572. doi: 10.1371/journal.ppat.1009572

Fig 4. EDA16 alters nucleosome positioning and expression of flg22-regulated genes.

Fig 4

(A) The EDA16 mutation alters flg22-induced nucleosome positioning. Venn diagram illustrating the overlap between genes (protein-coding genes plus 1000 nucleotides upstream their Transcription Start Sites, TSS) with at least one Differentially Positioned Nucleosome (DPN) in Col-0 (black), eda16-OE (blue) and eda16-ΔHc (red) 2 hours after elicitation with 100 nM flg22. (B) flg22-induced genes have distinct nucleosome occupancies in the eda16-OE and eda16-ΔHc mutants. Average of the nucleosome occupancy differences between flg22-treated and mock-treated Col-0 (black), eda16-OE (blue), and eda16-ΔHc (red) for flg22-induced genes. The graph is centred on the +1 nucleosome from the gene TSS. (C) The effect of EDA16 mutation on the flg22 response at the transcriptomic level. Venn diagram illustrating the overlap between flg22-regulated, Differentially Expressed Genes (DEGs) in Col-0 (black), eda16-OE (blue) and eda16-ΔHc (red) 2h after elicitation with 100 nM flg22.