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. 2021 May 20;17(5):e1009573. doi: 10.1371/journal.pgen.1009573

Fig 4. The sphingomyelin synthase SMS-5 mediates DAF-16 activation upon let-607 knockdown.

Fig 4

(A) Effects of sms-5 RNAi on let-607 RNAi-induced MTL-1::GFP expression. Left panel, representative images. Right panel: quantification data. Scale bar = 100 μm. (B) Effects of let-607 RNAi on the sms-5 mRNA expression. n = 3 per group. (C) Effects of sms-5 RNAi on let-607 RNAi-induced DAF-16::GFP nuclear occupancy. Left panel, representative images. White arrow indicates nuclear GFP signal. Right panel: semi-quantification data. Number of animals (n): control RNAi (97), let-607 RNAi (87), sms-5 RNAi (80) and sms-5 + let-607 RNAi (48). Scale bar = 25 μm. (D) Influence of choline on MTL-1::GFP expression in let-607 RNAi-treated animals. (E) MTL-1::GFP expression in WT and let-607 knockdown animals after sams-1 RNAi. Upper panel, representative images. Lower panel: quantification data. Scale bar = 100 μm. Data were presented as mean ± SEM. *** p < 0.001.