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. 2021 May 13;148(9):dev198796. doi: 10.1242/dev.198796

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© 2021. Published by The Company of Biologists Ltd

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Fig. 10. — Toy/Pnt are required during the second larval instar to suppress formation of the second antenna. (A-D) In Toy/Pnt knockdown discs, the second antenna initiates its development during the middle of the third larval instar. As with Toy/NURF double knockdown discs, wg expression is activated within the dorsal head epidermis at 96 h AEL. (E-L) Controlling the expression of the pnt RNAi lines during development indicates that Toy/Pnt are required during the second larval instar stage. Toy/ISWI and Toy/E(bx) are required during the same developmental window. Red, F-actin; green, Wingless. Scale bar: 50 µm.