Figure 3.

MSC-EXs could promote the expression of miR-145-5p in spinal cord tissue and inhibit the activation of TLR4/NF- κB pathway in SCI rats

(a) QRT-PCR was used to detect the expression of miR-145-5p in injured spinal cord tissue of rats in the Sham, Model, Model+EX-miR-145-5p-NC, Model+PBS and Model+EX-miR-145-5p groups. (b) QRT-PCR and Western blot (c–f) were used to detect the expressions of TLR4, p-P65, p65, p-IκBα and IκBα in the spinal cord tissues of rats in each group. (n = 3, ***P < 0.001, vs. Sham group; ^#P < 0.05, ^###P < 0.001, vs. Model+EX-miR-NC group; ^{^^}P < 0.01, ^{^^^}P < 0.001, vs. Model+PBS group)