Figure 8.

TLR4 overexpression significantly reversed the effect of EX-miR-145-5p on inhibiting inflammatory response and activating TLR4/NF- κB pathway in PC12 cells

(a) ELISA was used to detect TNF-α expression in PC12 cells transfected with miR-145-5p mimic and TLR4 overexpression vector. (c) ELISA was used to detect IL-1β expression in PC12 cells transfected with miR-145-5p mimic and TLR4 overexpression vector. (c) ELISA was used to detect IL-6 expression in PC12 cells transfected with miR-145-5p mimic and TLR4 overexpression vector. (d) The expressions of p65 and IκBα were detected by qRT-PCR in PC12 cells transfected with miR-145-5p mimic and TLR4 overexpression vector. (e–h) The expressions of p65 and IκBα were detected by Western blot in PC12 cells transfected with miR-145-5p mimic and TLR4 overexpression vector. (n = 3, ***P < 0.001, vs. LPS group; ^{^^}P < 0.01, ^{^^^}P < 0.001, vs. LPS+EX-miR-NC group)