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. 2021 Jun 1;10(1):1002–1015. doi: 10.1080/22221751.2021.1931466

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Characterization of Sad23L-nCoV-S and Ad49L-nCoV-S vaccines. (A) Recombinant adenovirus constructs Sad23L-nCoV-S and Ad49L-nCoV-S carrying the full-length S gene of SARS-CoV-2 under CMV promotor regulation within the deleted E1 region of Sad23L or Ad49L vector. (B) Western blot analysis for the expression of S protein from Sad23L-nCoV-S or Ad49L-nCoV-S infected HEK-293A cell lysates by rabbit polyclonal antibody to RBD. Sad23L-GFP or Ad49L-GFP virus infected cells were used as mock controls. (C) Expression of S protein in HEK-293A cells detected by immunofluorescence staining. (D) Seroprevalence of neutralizing antibody (AdNAb) to Ad5, Ad49L or Sad23L vector in 600 healthy blood donors.