Figure 4:

Fatty acid GUVs can encapsulate a wide range of materials. Materials encapsulated include a. pyranine (1 mM), b. 30-nm-diameter Nile Red dyed latex nanoparticles (0.1% w/v), and c. 400-nm-diameter polystyrene latex beads (0.1% w/v; see also Video S13). d–e. Oleic acid vesicles can encapsulate RNA (the same RNA oligomer as in Fig. 1a) and divide upon exposure to oleate micelles pipetted nearby. The time shown represents the number of seconds after injecting oleate micelles. Scale bar represents 10 μm for all images.