Figure 1. UC pouch stool has diminished secondary bile acids and altered microbial composition, which is associated with lower expression of bai genes, and less efficiency to convert primary bile acid to secondary bile acid in vitro than FAP pouch.

(A) Levels of DCA and LCA are significantly lower in UC versus FAP pouches, whereas CDCA is significantly higher measured by LC-MS. (B) Total bacterial species present (at 10,000 reads per sample) show reduced richness and Shannon diversity in UC pouch microbiota. (C) Bray-Curtis distances to assess β-diversity at community level differences between UC pouch and FAP pouch, as measured by PERMANOVA test. (D) Relative proportions of most abundant species revealed reduced taxon abundance of Firmicutes Ruminococcaceae in UC compared to FAP pouches. (E) Bai A-K gene transcription (established by RNA-Seq) is lower in UC pouches compared to FAP pouches. (F) Bai CD gene expression, a key enzyme in SBA biosynthesis, is also lower in UC pouches compared to FAP pouches. (G) The stool from UC pouch was less efficient in converting exogenous CA and CDCA to DCA and LCA respectively than FAP pouch. Data represented as mean ± SEM. (A) UC pouch n=17, FAP pouch n=7, * P<.05, two-tailed t-test, post multiple t-test with Benjamini and Hochberg posttest correction, (B) UC pouch n=11, FAP pouch n=5; * P<.05, two-tailed t-test, (C) UC pouch n=11, FAP pouch n=5, Permanova test, R²=.093, (D) UC pouch n=11, FAP pouch n=5, ** P<.01, two-tailed t-test, (E) UC pouch n=7, FAP pouch n=3, *** P<0.001, two-tailed t-test, (F) UC pouch n=7, FAP pouch n=3, * P<0.05, two-tailed t-test, (G) UC pouch n=17, FAP pouch n=7, ** P<.01, two-tailed t-test.