Skip to main content
. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Dev Biol. 2021 Mar 26;476:137–147. doi: 10.1016/j.ydbio.2021.03.019

Figure 7: Sustained MEK/MAPK activity in the cortex alters normal morphology but does not induce LGE or OPC genes at mid-gestation stages.

Figure 7:

Representative images at E15.5 from controls (A, C, E, G, I) and MEK/MAPK GOF double transgenic Emx1-Cre;RosaMEK1DD/+ (B, D, F, H, J) embryos. p-ERK1/2 and Etv5 expression are increased in the VZ of cortical MEK/MAPK GOF embryos (compare B,D to A,C). Cortical MEK/MAPK GOF embryos showed an enlarged ventricle (compare B to A). Gsx2 expression was not detected in the cortex of MEK/MAPK GOF embryos (compare F to E). Sp8 expression was increased in the cortical VZ (compare H to G). Red asterisk refers to dorsal medial area of cortical Sp8 expression in controls (G) and expanded expression in MEK/MAPK GOF embryos (H). The OPC marker Pdgfrα was not detected in the cortex of MEK/MAPK GOF embryos (J). Arrow in J refers to clustering of migrating ventral Pdgfrα OPCs that are not observed in controls (I). Scale bars in: B= 200μM for images in A-J.