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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2021 Mar 15;20(6):1062–1071. doi: 10.1158/1535-7163.MCT-20-0807

Figure 2. Androgen responsive TNBC PDX model.

Figure 2.

(A) TNBC PDX HCI-009 was propagated in NSG mice. Tumor tissue was stained for AR by IHC. AR staining in MDA-MB-453 cells is shown for comparison. (B-E) HCI-009 PDX tumor portions were injected into the mammary fat pads of female NSG mice supplemented with cellulose, as a control, or DHT (dihydrotestosterone; 8mg/pellet). (B) Tumor volume was measured with calipers. (C) AR was stained by IHC. (D) IHC staining of known AR-regulated genes. Quantification of staining is presented as an IHC score, combining cell positivity and staining intensity. (E) Shown are the top 30 up- and downregulated genes identified by RNAseq in HCI-009 PDX tumors from mice treated with DHT. Mean ± standard error of the mean; * p<0.05, ** p<0.01, *** p<0.001; scale bars = 50μm.