Figure 4. The association between high AR expression and cell cycle pathway activation.

scRNAseq was performed on untreated MDA-MB-453 cells cultured in full serum media. Cells were divided into groups based on their expression of AR, AR^Low and AR^High. (A) Histogram of the distribution of AR expression in single MDA-MB-453 cells including AR level group determination. (B) Ingenuity pathway analysis was performed on genes differentially expressed between AR^Low and AR^High groups. Shown are the top 15 most significantly altered disease and function pathways. (C) Differential expression of genes involved in cell cycle indicating cell cycle activation associated with high AR expression, shown as 10 cell aggregates.