. 2021 Apr 17;48(4):3237–3244. doi: 10.1007/s11033-021-06328-7

Table 1.

Primers sequence, annealing temperature, products length and the percentage of the gel necessary to analyze polymorphisms of the LEP and LEPR genes

Primers	Primers sequence	Primer binding temperature [^oC]	Amplicon length [bp]	Agarose gel [%]
LEP F LEP R	5′-TTTCTGTAATTTTCCCGTGAG-3′ 5′-AAAGCAAAGACAGGCATAAAAA-3′	53	242	1,5
LEPR 109Lys>Arg F LEPR 109Lys>Arg R	5′-TTTCCACTGTTGCTTTCGGA-3′ 5′-AAACTAAAGAATTTACTGTTTGAAACAAATGGC-3′	55	101	3
LEPR 223Gln>Arg F LEPR 223Gln>Arg R	5′-AAACTCAACGACACTCTCCTT-3′ 5′-TGAACTGACATTAGAGGTGAC-3′	59	80	3

Primers

Primers sequence

Primer binding temperature [^oC]

Amplicon length [bp]

Agarose gel [%]

LEP F

LEP R

5′-TTTCTGTAATTTTCCCGTGAG-3′

5′-AAAGCAAAGACAGGCATAAAAA-3′

242

1,5

LEPR 109Lys>Arg F

LEPR 109Lys>Arg R

5′-TTTCCACTGTTGCTTTCGGA-3′

5′-AAACTAAAGAATTTACTGTTTGAAACAAATGGC-3′

101

LEPR 223Gln>Arg F

LEPR 223Gln>Arg R

5′-AAACTCAACGACACTCTCCTT-3′

5′-TGAACTGACATTAGAGGTGAC-3′

F forward primer, R reverse primer, bp base pair