KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-METTL3 | Abcam | ab195352 |
| Anti-METTL3 | Invitrogen | MA5-27527 |
| Anti-m6A | Abcam | ab151230 |
| Anti-c-Myc | Abcam | ab185656 |
| Anti-c-Myc | Sigma | OP10-200UG |
| Anti-Avpr2 | EMD | AB1797P |
| Anti-Puromycin | Developmental Studies Hybridoma Bank | PMY-2A4 |
| Anti-HA | Cell Signaling | #3724 |
| Anti-PCNA | Santa Cruz | sc-7907 |
| Anti-pHH3 | Sigma | H0412 |
| Anti-pCreb1 | Cell Signaling | 9198S |
| Anti-Yap | Cell Signaling | #4912 |
| Anti-GFP | Aves | GFP-1020 |
| Anti-DBA | Vector Laboratories | B-1035 |
| Anti-Mettl14 | Sigma | HPA038002 |
| Bacterial and Virus Strains | ||
| Ad-CMV-iCre virus | Vector Biolabs | #1045 |
| Biological Samples | ||
| Normal human and ADPKD kidney samples | PKD Biomarkers and Biomaterials Core in the Kansas PKD Center at the Kansas University Medical Center (KUMC) | |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Puromycin | Invitrogen | A1113803 |
| O-Propargyl-Puromycin | JenaBioScience | NU-931-5 |
| 8-Br-cAMP | Sigma | B7880 |
| alamarBlue | Invitrogen | DAL1025 |
| methionine | Sigma | M5308 |
| s-adenosylmethionine | Cayman Chemicals | 13956 |
| s-adenosylhomocysteine | Cayman Chemicals | 13603 |
| Critical Commercial Assays | ||
| m6A elisa kit | Epigentek | P-9005-96 |
| SAM elisa kit | Cell Biolabs | STA-671-C |
| Magna MeRIP™ m6A Kit | EMD Millipore | 17-10499 |
| Submitted data | ||
| MeRIP-seq | NCBI Gene Expression Omnibus | GSE165956 |
| RNA-seq | NCBI Gene Expression Omnibus | GSE165956 |
| Experimental Models: Organisms/Strains | ||
| Ksp/Cre | Shibazaki et al., 2008 | N/A |
| Ksp/rtTA | Pan et al., 2013 | N/A |
| tetO-Cre | Jackson Laboratories | Stock No: 006234 |
| Pkd1F/F | Jackson Laboratories | Stock No: 010671 |
| Pkd1RC/RC(PKD1 p.R3277C) | Hopp et al., 2012 | N/A |
| Mettl3F/F | UT Southwestern Med Ctr | N/A |
| Mettl3Tg | UT Southwestern Med Ctr | N/A |
| mIMCD3 cells | ATCC | CRL 2123 |
| Oligonucleotides | ||
| siRNA against Mettl3 (UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, UGGUUUACAUGUUUUCCUA) | Dharmacon | L-049446-01-0020 |
| Scrambled siRNAs (ACAGCAGGCACAGACAGGCAGU) | Dharmacon | D-001810-10-20 |
| Recombinant DNA | ||
| Plasmid - CAG-LSL-Mettl3cDNA-HA/HA/HA-IRES-GFP | This paper | N/A |
| Plasmid - pLightSwitch Empty (pLS) 3′-UTR | Switchgear Genomics | S890005 |
| Plasmid - pGL3 | Promega | E1751 |
| Software and Algorithms | ||
| Imagej | NIH | https://imagej.nih.gov/ij/ |
| Zen blue software | Zen | https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html |
| ImageLab | Bio-rad | https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z |
| Ingenuity Pathway Analysis (IPA) | Qiagen | https://analysis.ingenuity.com/pa/installer/select |
| Prism | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| Integrative Genomics Viewer (IGV) | Broad Institute | http://software.broadinstitute.org/software/igv/ |