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. 2021 Jun 2;4:658. doi: 10.1038/s42003-021-02175-1

Fig. 7. PP2A deletion in LepR+ MSCs increases Runx2 phosphorylation at Ser472 and reduces chondrocyte hypertrophy.

Fig. 7

MSCs isolated from WT (WT MSCs) and Lepr-cre; Ppp2r1a fl/fl mice (PP2A KO MSCs) were subjected to analysis. a Representative western blot analysis of WT MSCs after osteogenic induction. b Representative western blot analysis of WT MSCs after adipogenic induction. c Western blot analysis of WT and PP2A KO MSCs. d Co-Immunoprecipitation study of cell lysates from WT MSCs with indicated induction for osteogenesis. IP with PP2A-specific or phospho Ser 472 Runx2-specific antibody and precipitates were probed for phospho Ser472 Runx2 and PP2A. e and f IHC analysis of inactive form phospho Ser472 Runx2 expression in hypertrophic chondrocyte of POC (E18.5) and SOC (P15) from Lepr-cre; Ppp2r1a fl/fl mice and relative WT littermate mice. g Quantification of phospho Ser472 Runx2 percentage in hypertrophic chondrocyte of POC (E18.5) and SOC (P15) from Lepr-cre; Ppp2r1a fl/fl mice and relative WT littermate mice. ***p < 0.001 as determined with Student’s t-test. Data are mean ± s.d. h Scheme representing regulation of MSC differentiation by PP2A and its substrates protein. Scale bar, 50 μm.