Skip to main content
. 2021 Jun 2;12:3291. doi: 10.1038/s41467-021-23597-9

Fig. 3. The overexpression of Drosophila CG11070 and its mammalian orthologue UBE4B alleviated hTau phenotypes in Drosophila.

Fig. 3

a, b Eye-specific overexpression of Drosophila CG11070 or its mammalian orthologue UBE4B in GMR > hTau flies increased eye size relative to GMR > hTau controls. N = 5 biologically independent experiments. In the box plots the whiskers represent the 5th to 95th percentile range. c, d Neuronal overexpression of Drosophila CG11070 or its mammalian orthologue UBE4B using Elav-Gal4 in Elav > hTau flies significantly increased larval crawling. N = 10 biologically independent experiments. In the box plots the whiskers represent the 5th to 95th percentile range. e, f Neuronal overexpression of Drosophila CG11070 or its mammalian orthologue UBE4B using Elav-Gal4 in Elav > hTau flies significantly increased synaptic bouton numbers relative to Elav > hTau controls. Scale bar 100 µm. N = 10 biologically independent experiments. In the box plots the whiskers represent the 5th to 95th percentile range. g–i Western blotting revealed that ocular overexpression of Drosophila CG11070 or its mammalian orthologue UBE4B in GMR > hTau flies significantly reduced total and phosphorylated Tau protein levels relative to GMR > hTau controls. N = 4 biologically independent experiments. Data are presented as the mean ± s.e.m. Statistical significance was determined with a two-tailed Student’s t-test. Statistical source data.