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. 2021 Jun 2;11:11585. doi: 10.1038/s41598-021-91129-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Workflow of the study. Comparing different total EV isolation methods for protein characterization to proceed with surface protein-specific EV capture from cell culture supernatant. The antibody microarray analysis of surface EV marker can serve as a tool to rapidly investigate targets for specific isolation. Selecting tissue- or disease-derived EVs can be applied in EV diagnostics.