FIGURE 4.

Validation of PCBP2 knockdown effect on splicing. Validation by qPCR of three genes identified by RNA-Seq to undergo transcript switching upon silencing of PCBP2. Transcription of reference exons of each of the genes was used for normalization. The specific included or excluded exons are depicted in the diagrams which also indicate the location of the primers used (forward [F] and reverse [R]). FLNB shows significant inclusion of exon 30 upon PCBP2 silencing, while NCOR2 and EXOC7 show significant exclusion of exon 8 and 7, respectively. Experiments were repeated three times using 3–4 replicates per treatment. Data are presented as mean ± SD and tested with a Student’s t-test. ** Indicates p < 0.01; *** indicates p < 0.001.