Table 1.

The primary outputs from Begonia and where to find them.

Output	Description
ROA event table	Each row represents one event. The table contains the center position, start frame, end frame, duration, size and duration. Saved with the key-value pair “roa_table” after processing.
ROA traces	Time series of roa frequency (new events per frame) and ROA density (active x−y−t voxels) key-value pair “roa_traces” after processing.
ROA mask	A binary 3D matrix representing the imaging time series, where 1’s represent detected events. Saved with the key-value pair “roa_mask_chx” (x denoting the channel where ROAs have been detected).
ROA 3D plot	A GUI is provided to produce 3D ROA plots as in Figure 5B.
ROI table	A table containing the manually segmented ROIs. Each row represents one ROI and contains the size, location, channel, name and a unique identifier of the ROI. Saved with the key-value pair “roi_table.”
ROI traces	Raw and ΔF/F0 normalized signals from ROIs. Saved with the key-value pair “roi_signals_raw” and “roi_signals_dff.”
ROI active pixels	Fraction of ROI that has a ROA per time unit. Saved with the key-value pair “roi_signals_raw” and “roi_signal_rpa.”