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GCP induces autophagy in HeLa cells via the PI3K/Akt/mTOR signaling pathway. (A) Flow cytometry assay to detect autophagy level using by MDC staining, *p < 0.05, **p < 0.01 vs control. (B) Acridine orange staining was used to detect autophagic vacuoles. (C) The level of autophagy-related protein and (D) PI3K/Akt/mTOR signaling pathway protein expression shown by western blot analysis.
