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Inhibition of autophagy suppresses apoptosis induced by GCP. HeLa cells were preincubated with or without 3-MA for 2 h, then treated with GCP (1500 nM) for 24 h. (A) The CCK8 assay was used to measure the cell viability. (B) The levels of LC3, Cleaved-caspase 3, Cleaved PARP were detected by western bolt analysis. (C, D) Detection of apoptosis by flow cytometry. Each bar represents as mean ± SD from three independent experiments. **p < 0.01 vs control.
