Figure 7.

Isolated C42 and C99 derived hexameric Aβ do not readily nucleate monomeric Aβ40 (mAβ40). (a) 50 µM mAβ40 was seeded with 5- or 10% hexamer and the solution was then incubated with 20 µM ThT. Fluorescence was monitored over 4 h. (b) Addition of both 5- and 10% did not result in an increased fluorescence at early time points (0–2 h) (c) Western blotting detected with the W0-2 antibody revealed seeding conditions with both hexamers does not lead to the formation of higher molecular weight assemblies compared to mAβ40 only. Dashed borders indicate the membrane was cut before detection.