Figure 5.

Phylogenetic analysis of aminoglycoside (2′)-N-acetyltransferase substrate specificity. (a) Percent conservation of key residues involved in aminoglycoside and plazomicin binding, where conservation is colored according to the legend. (b) Predicted classification of 61 identified sequences as binders, likely binders, unlikely binders, and non-binders for aminoglycosides or plazomicin, colored according to the legend. (c) Phylogenetic tree of identified sequences colored as in panel (b), with aminoglycosides’ coloring in the center, and plazomicin coloring in the outer circle. The original five query sequences are labelled, and plasmid-encoded sequences are highlighted with an asterisk (*).