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. 2021 Jun 2;7:48. doi: 10.1038/s41522-021-00219-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a P. aeruginosa biofilm protocol for the eardrum-mimicking membrane. b Final diagram of the biofilm phantom and the direction of microplasma jet array treatment. c Photo of an empty glass bottom cell culture dish. d Photos of the membrane immediately after loading the bacterial suspension. e Photos of 4-day-old biofilm on the membrane.