Fig. 5. The gHgL mAbs bind to diverse epitopes on the gHgL.

a The binding sites of four gHgL reference antibodies: 3–16 binds to the gH of pentamer, 13H11 binds to gHgL kinked region, 3G16 binds to the C-terminal domain of gH, MSL-109 binds to the H2 domain of gH as indicated. b Forty-four gHgL mAbs bind to overlapping regions with the four reference mAbs: 66% to the 3–16 epitope, 23% to the 13H11 epitope, 9% to MSL-109 epitope, and 2% to the 3G6 epitope. c, d Neutralization potency (IC₅₀) of the gHgL mAbs in ARPE-19 cells (c), and MRC-5 cells (d), positively correlates with their binding affinity (EC₅₀) to the HCMV virion. The IC₅₀ and EC₅₀ values shown are representative of two independent experiments. The linear association was calculated by the Pearson correlation, two-tailed. The Molecular graphic images of pentamer (PDB: 5VOD) were produced using UCSF ChimeraX (http://www.cgl.ucsf.edu/chimerax/).