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. 2021 Jun 2;11:11659. doi: 10.1038/s41598-021-91293-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SOG1 is important in controlling cell cycle progression and entry into endocycle in response to salinity stress. (A–C) Detection of protein accumulation levels of key regulators of cell cycle progression and endoreduplication including WEE1 (A), CCS52A (B) and E2Fa (C), respectively in 7-days old OE-1, wild-type and sog1-1 seedlings exposed to increasing concentrations of NaCl for 12 h. The blots were cut prior to incubation with the respective primary antibody. Loading control of total protein extracts has been presented in the lowermost panel. Representative gel blot images from at least three independent experiments are shown. Replicates of the protein gel blots have been presented in Supplementary Fig. S16. (D–F) Quantification of band intensity for determining the accumulation levels of WEE1 (D) CCS52A (E), E2Fa (F) proteins as detected by immunoblotting. Densitometry analysis was carried out by ImageJ software (NIH). Error bar indicates standard deviation. Asterisks indicate significant statistical differences (*p < 0.05, **p < 0.01) from control (without NaCl treatment) using one-way ANOVA factorial analysis.