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. 2021 Jun 2;11:11659. doi: 10.1038/s41598-021-91293-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Salinity induced endoreduplication and nuclear area expansion. (A–C) Scatter plots showing DNA ploidy distribution in root tip nuclei of 7-days old OE-1, wild-type and sog1-1 mutant plants treated without or with 50 and 150 mM NaCl. (D) Confocal imaging of DAPI-stained nuclei in the root cells of 7-days old OE-1, wild-type, sog1-6 and sog1-1 mutant seedlings (first, second, third and fourth panel, respectively); Scale bar: 10 μm. (E) The nuclei in a pair of wild-type stomata cells represent 2C. Strongly stained dots in each nucleus correspond to centromeres. (F) Measurement of in-situ fluorescence in DAPI-stained root tip nuclei of wild-type, OE-1, sog1-6 and sog1-1 mutant seedlings. Each bar represents the mean value of three independent replicates. Error bar indicates standard deviation. Asterisks indicate significant statistical differences (*p < 0.05, **p < 0.01) from control (without NaCl treatment) using one-way ANOVA factorial analysis.