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. 2021 Jun 2;11:11659. doi: 10.1038/s41598-021-91293-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Salinity induced cell area expansion in the root tip epidermal cells. (A–D) Propidium iodide stained root tip cells of 7-days old OE-1, wild-type, sog1-6 and sog1-1 mutant seedlings respectively, either treated without or with 50 and 150 mM NaCl. Red arrows showing increase in cell size. Scale bar: 50 μm. (E–L) Measurement of area of root tip cells situated between 40 and 200 μm from the quiescent centre (QC) using ImageJ software (NIH). Regression lines included in (E–L); For OE-1 (E,F), R² = 0.421 (Control), 0.598 (50 mM), 0.787 (150 mM); for wild-type (G,H), R² = 0.507 (Control), 0.617 (50 mM), 0.713 (150 mM); for sog1-6 (I,J), R² = 0.228 (Control), 0.236 (50 mM), 0.354 (150 mM) and for sog1-1 (K,L), R² = 0.181 (Control), 0.191 (50 mM), 0.204 (150 mM). The F value was found to be < 0.001 for all regression analyses.