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. 2021 Jun 2;4:666. doi: 10.1038/s42003-021-02114-0

Fig. 6. NCLX-KO slices fail to exhibit hippocampal Schaffer-collateral LTP.

Fig. 6

a Schematic representation of extracellular recordings (fEPSP) from Schaffer collateral synapses in the CA1 area of acute hippocampal slices. The high-frequency stimulation (HFS) LTP induction protocol (5 pulses at 100 Hz, repeated once after 20 s) is shown below. b fEPSPs were evoked every 15 s, responses were normalized by baseline values. The CA3 area was stimulated to evoke fEPSP of ~0.3 mV. After 5 min of baseline recording, LTP was induced by HFS (arrow). Shown are mean ± SEM responses in WT (n = 18 slices from 7 mice) and NCLX-KO (n = 9 from 4 mice). c LTP in NCLX-KO slices is abolished. LTP during the horizontal bar in (b), n = 18 and 9 recordings, respectively, *p = 0.029, Mann–Whitney u test (U = 124, Z = 2.19), bars indicate medians and the error bars the 10–90 percentiles.