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. 2021 Jun 2;11:11562. doi: 10.1038/s41598-021-91107-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Potent inhibition of α1AT translocation into the ER by Ipom-F correlates with cytotoxicity. (a) HepG2 cells treated for 24 h with increasing concentrations of Ipom-F were analysed by immunoblotting for endogenous α1AT. The positions of non-glycosylated (0ψ) and N-glycosylated α1AT (ψ; ER and post-ER species) are indicated. Representative immunoblots shown (full-length blots presented in Supplementary Fig. S6). Infrared fluorescence-based quantification of the efficiency of α1AT translocation into the ER is indicated below the gel. The percentage of α1AT translocation was calculated by dividing the normalised signal intensity of the N-glycosylated species by the sum of N-glycosylated and non-glycosylated species within each lane. (b) Dose–response curves for the effect of 24 h Ipom-F treatment on the translocation of endogenous α1AT into the ER (calculated as in (a)) and cell viability measured using the MTT assay. Curves are means ± s.e.m of three independent experiments (performed in triplicate for the MTT assay). IC50 (half-maximal effective concentration) values of the dose-responses are shown.