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. 2021 Jun 2;12:3281. doi: 10.1038/s41467-021-23531-z

Fig. 1. Development of synthetic reproductive barriers.

Fig. 1

a Homozygous dCas9-VPR flies were crossed bidirectionally to homozygous sgRNA flies. Shaded gray background indicates expected lethal crosses. b Homozygous dCas9-VPR individuals were crossed bidirectionally to heterozygous spCas9/sgRNA individuals. Surviving individuals were repeatedly inbred to generate SPECIES. c, d Plots depicting % progeny survival from the crosses depicted in A and B. N = 3 biologically independent replicates for each gRNA combination (eight total combinations). Middle lines indicate mean. Gray dots indicate homozygous sgRNA and heterozygous spCas9/sgRNA flies crossed to wildtype flies. Light and dark blue dots indicate homozygous sgRNA and heterozygous spCas9/sgRNA flies crossed to homozygous dCas9-VPR mothers and fathers, respectively. Purple stars indicate the presence of maternally deposited Cas9. Source data are provided as a Source Data file.