Figure 5.
Effects of dietary thiamine supplementation on the expression and distribution of tight junction proteins during HC diet feeding. (A) Immunohistochemistry analysis of ZO-1, claudin-1, claudin-4, and occludin in the ruminal epithelium. (B) Positive rate analysis. All scale bars represent 100 μm (n = 8 goats/treatment). CON, low-concentrate diet; HC, high-concentrate diet; HCT, high-concentrate diet supplemented with 200 mg of thiamine/kg of dry matter intake. The mean values in columns without a common superscript letter differ (P < 0.05).