Fig. 3.

A multiplex LAMP-BEAC method assaying four amplicons. Assays were carried out using a LAMP primers and molecular beacon to detect human STATH RNA (a) and three primer sets and corresponding beacons to detect SARS-CoV-2: E1 (b), As1e (c), and Penn (d). For these assays, synthetic SARS-CoV-2 RNA was diluted into the saliva (inactivated as described [9]); copies per reaction are shown by the color code below the figures. a–d Amplification curves of the reactions showing fluorescent intensity (x-axis) over time (y-axis). e–h Endpoint melt curves of the reactions shown in a–d showing changes in fluorescent intensity (y-axis) as the temperatures (x-axis) of the final reaction products were raised from 25 to 95°C