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. 2021 May 20;9:675517. doi: 10.3389/fcell.2021.675517

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Copyright © 2021 Crouzier, Diez, Richard, Cubedo, Barbereau, Rossel, Delaunay, Maurice and Delprat.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of activated Caspase-3 positive cells in pde6a^Q70X zebrafish during development. (A,B) Representative confocal images of the retina of pde6a^WT and pde6a^Q70X zebrafish obtained after immunostaining of the retinal section with activated Caspase-3 antibody (red) at 5 dpf (A) and 21 dpf (B). Nuclei were highlighted with DAPI (blue). Scale bar = 30 μm. (C,D) Quantification of the number of activated Caspase-3 positive cells in the entire retina based on three independent measurements of larvae per group at 5 dpf (C) and 21 dpf (D). The number of animals is indicated within the columns. ** p < 0.01, ***p < 0.001.