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. 2021 May 20;9:669696. doi: 10.3389/fcell.2021.669696

FIGURE 2.

FIGURE 2

NLRC5 knockdown effectively ameliorates retinal damage following ischemia–reperfusion. (A) RT-qPCR analysis of retinal NLRC5 gene knockdown efficiency using a targeted siRNA (n = 6). (B) HE staining of mouse retina showing that intravitreal injection of NLRC5 siRNA ameliorates tissue damage and reverses the reduction in retinal thickness following ischemia–reperfusion compared to retinas transfected with control siRNA (Scale bar: 30 μm, Magnification: ×400, n = 4). (C) Retinal immunofluorescence images and corresponding quantitative analysis of anti-RBPMS-labeled RGCs showing that the reduction in RGC number following RIR is reversed by NLRC5 knockdown (Scale bar: 50 μm, Magnification: ×200, n = 4). (D) TUNEL staining of retina following RIR (Scale bar: 50 μm, Magnification: ×400, n = 4). The white arrow indicates TUNEL-positive cells. All samples were obtained on the third day of reperfusion. I/R, retinal ischemia–reperfusion; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Results are presented as mean ± SD. *P < 0.05, **P < 0.01. Two-way ANOVA with post hoc Bonferroni tests was applied.