FIGURE 5.

Detection of Arabidopsis myrosinases in the feces of P. armoraciae. (A) One dimensional SDS/PAGE gel of crude feces protein extract. Numbers indicate samples excised for proteomic analysis by nano-UPLC-MS^E (only odd numbers are shown). TGG1-derived peptides were detected in bands marked with asterisks (*). (B) Amino acid sequence alignment of the Arabidopsis myrosinases TGG1 (AT5G26000.1) and TGG2 (AT5G25980.2). Identical amino acids in the TGG2-sequence are represented by a dot. Peptides highlighted with black background were detected by nano-UPLC-MS^E (Supplementary Table 6). Only one detected peptide matched both TGG1 and TGG2.