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. 2021 May 20;12:627986. doi: 10.3389/fimmu.2021.627986

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Copyright © 2021 Grönwall, Liljefors, Bang, Hensvold, Hansson, Mathsson-Alm, Israelsson, Joshua, Svärd, Stålesen, Titcombe, Steen, Piccoli, Sherina, Clavel, Svenungsson, Gunnarsson, Saevarsdottir, Kastbom, Serre, Alfredsson, Malmström, Rönnelid, Catrina, Lundberg and Klareskog

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Screening of RA B cell-derived monoclonal antibodies show acetyl-lysine and carbamylation reactivity only in CCP2+ clones. (A) Recombinant monoclonal antibodies derived from single B cells from RA patients were screened for reactivity to carbamylated and citrullinated antigens by ELISA at 5µg/ml IgG. 250 clones had no known citrulline reactivity (others) and 16 clones were positive for binding in CCP2 ELISA (ACPA mAbs). Clones showing unspecific binding in any poly-reactivity assay were excluded before screening. (B) Reactivity pattern of 16 monoclonal anti-CCP2 positive ACPA clones to different modified antigens. Data is derived from the ACPA fine-specificity microarray (Cit-peptides) or ELISA (Carb/KAc and mod-Vim assays).