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. 2021 May 20;9:660851. doi: 10.3389/fcell.2021.660851

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Copyright © 2021 Eirin, Meng, Zhu, Li, Saadiq, Jordan, Tang, Lerman, van Wijnen and Lerman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of ASC-derived EVs and their daughter EVs. (A) Flow cytometry analysis of human ASCs. Cells were stained with anti-CD73, CD90, CD105, CD14, CD34, and CD45 antibodies. (B) ASC-derived EVs expressed common EV markers, including CD9, CD63, and CD81 by Western blotting. (C) Transmission electron microscopy showing ASC-derived lean- and obese-EVs exhibiting the classic “cup-like” morphology. (D) Representative size-distribution curve of lean- and obese-EVs by nanosight tracking analysis (NTA). (E) Quantification of EV concentration and size by NTA (Student’s t-test). *p < 0.05 vs. Lean-EVs.