Table 5. Physiochemical Property Assessment of the Primary Sequence of the Multi-epitope Vaccine Construct.
| parameters | result |
|---|---|
| number of amino acids | 196 |
| molecular weight | 21.40 kDa |
| theoretical pI | 9.41 |
| positively charged residues (Asp + Glu) | 12 |
| negatively charged residues (Arg + Lys) | 22 |
| extinction coefficient (at 280 nm in H2O) | 16,765 |
| estimated half-life (mammalian reticulocytes, in vitro) | 30 h |
| estimated half-life (yeast cells, in vivo) | >20 h |
| Estimated half-life (Escherichia coli, in vivo) | >10 h |
| instability index | 35.78 |
| aliphatic index | 92.14 |
| grand average of hydropathicity (GRAVY) | –0.094 |