Figure S1.

SARS-CoV-2 infection of HEK293T/ACE2/TMPRSS2 and A549/ACE2/TMPRSS2, related to Figure 1

(A) A549 cells expressing ACE2 and TMPRSS2 were infected with SARS-CoV-2 at MOI of 3 for 3, 6, 12, 18, and 24 hours. Fixed cells were incubated with a fluorescence antibody to the nucleocapsid and DAPI stain was used to label the nuclei. Immunofluorescent images were taken using an EVOS microscope with a 10x lens. Bars show mean ± SD (B) Similar to (A) for HEK293T cells. (C) Plaque assay confirming SARS-CoV-2 inactivation for HLA-IP experiments. A549 cells were infected with SARS-CoV-2 at MOI of 3 for 24 hours. 10-fold serial dilutions were prepared in Opti-MEM and used to infect Vero cells in a 24-wells plate. Comparing plaques in (left) cultured media of infected A549 cells; (middle) SARS-CoV-2 infected A549 cells treated with a lysis buffer containing 1.5% Triton-X and Benzonase for 3 hours; and (right) non-infected A549 cells. When adding the 1:10 dilution of the lysis buffer, infected and non-infected cells died immediately due to the relatively high Triton-X concentration.