Figure 3.
Synthetic, multiplex A. baylyi CRISPR arrays block acquisition of multiple genes. Cells containing no exogenous CRISPR arrays (WT), 4-spacer arrays targeting kan1 and kan2, and an 8-spacer array targeting both kan genes (x-axis tick labels) were incubated with linear, genomically integrating DNA. Donor DNA constructs included Vgr4-Kan1 (A), Vgr4-Kan2 (B), both kan constructs (C), or a nontargeted beta-lactamase gene (D). Data include two experimental replicates, each with three measurement replicates, error bars indicate propagated standard deviations (see Methods), and limits of detection were roughly 10−6. **p < 10−7.