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. 2021 Feb 24;70(5):1170–1184. doi: 10.2337/db20-1008

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© 2021 by the American Diabetes Association

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at https://www.diabetesjournals.org/content/license.

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Dock5 regulates keratinocyte migration, proliferation, and adhesion. Keratinocytes were transfected with Dock5-specific siRNA (Si-Dock5) or overexpression plasmid (Oe-Dock5). A: The transfection efficiency of overexpression and knockdown is presented. B: Live cell images were acquired 16 h after scratching in a wound-healing assay via a live cell imaging system. C: Wound recovery rate was quantified. D: Cell migration trajectory was tracked for 2 h at 8–10 h after scratching. The center indicates the start point, and magenta dots indicate end points. Each line shows the trajectory of a randomly chosen cell (left [L], right [R], forward [F], or opposite [O] of the direction of migration). E: Cell proliferation was assessed by Cell Counting Kit 8 assay. F: Cell adhesion was quantified using the CyQUANT Assay Kit. n = 3 for A–C and F, n = 65–75 for D, and n = 5 mice per group for E. Data are presented as means ± SEMs. *P < 0.05, ***P < 0.001. OD, optical density.