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. 2021 Feb 9;70(5):1070–1083. doi: 10.2337/db20-0679

Figure 2.

Figure 2

Virgin β-cells can proliferate. A: Two-dimensional representative image of a 3-week-old wild-type mouse islet immunostained with insulin (red), Ucn3 (white), EdU (green), and DAPI (blue). The cyan arrowhead points to a proliferating EdU-positive mature β-cell that expresses Ucn3, while the yellow arrowhead points to a proliferating EdU-positive virgin β-cell that lacks Ucn3 expression. B: Schematic of EdU injection timeline of mIns-H2b-mCherry × Ucn3-EGFP mice. C: Three-dimensional representative image of a 1-month-old mIns-H2b-mCherry × Ucn3-EGFP mouse islet stained for EdU (white) detection. Yellow arrowheads indicate mCherry single-positive dividing virgin β-cells. Cyan arrowheads indicate mCherry and EGFP double-positive dividing mature β-cells.